Pular para o conteúdo
Merck
Todas as fotos(2)

Documentos

MTOX1006P24

Sigma-Aldrich

BCRP/MRP2 Double Knockout Caco-2 Cells

human cervix, Epithelial

Sinônimo(s):

BCRP/MRP2 Double Knockout C2BBe1 Cells

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(2)

About This Item

Código UNSPSC:
41106514
NACRES:
NA.81

product name

BCRP/MRP2 Double Knockout Caco-2 Cells, one assay ready, 24 well plate

fonte biológica

human colon

forma

solid

morfologia

Epthelial

técnica(s)

drug transporter assay: suitable
permeability assay: suitable

aplicação(ões)

ADME/TOX

Procurando produtos similares? Visita Guia de comparação de produtos

Descrição geral

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The BCRP/MRP2 double knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the BCRP and MRP2 efflux transporters.

The three week production lead time begins on the Monday following a purchase, in the third week the plates are shipped on Tuesday for receipt on Wednesday or Thursday. As a biologic product that′s shipped at room temperature the cells must be processed immediately upon receipt.

Aplicação

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Características e benefícios

The Caco-2 subclone, C2BBe1 cells, are ideal for transporter analysis as they express multiple transporters, are human derived and grow in a homogenous monolayer that forms tight juntions which is necessary for efflux ratio analysis. Other benefits include:

  • Functional knockout of the BCRP and MRP2 genes eliminates the reliance on chemical inhibitors to determine if a compound is a BCRP or MRP2 substrate
  • The 24 well Transwell format enables the BCRP and MRP2 knockout cells to be included in standard drug transporter protocols
  • Human assay with no interference from animal inhibitors
  • Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

M J Briske-Anderson et al.
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 214(3), 248-257 (1997-03-01)
The Caco-2 cell line is used by many investigators as a model of the intestinal epithelium to study nutrient uptake and transport. Our goal was to create an awareness of inherent variabilities in the Caco-2 cell line which may influence
Mark I Kaldas et al.
The Journal of pharmacy and pharmacology, 55(3), 307-312 (2003-05-02)
Resveratrol is a dietary constituent suggested to have protective effects against cancer as well as cardiovascular disease. The purpose of the study was to learn whether this agent could be absorbed in man and enter the systemic circulation. This was
V Pade et al.
Journal of pharmaceutical sciences, 87(12), 1604-1607 (1999-04-03)
The objective of this investigation was to establish a relationship between drug permeability and solubility in vitro and the extent of drug absorption in humans. We selected drugs with varying permeabilities and solubilities with the aim of establishing a relationship
S Yee
Pharmaceutical research, 14(6), 763-766 (1997-06-01)
To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous
X Wu et al.
Pharmaceutical research, 17(2), 209-215 (2000-04-06)
The purpose of this study was to elucidate the mechanisms by which an HMG-CoA reductase inhibitor, atorvastatin (an organic acid with a pKa of 4.46), was transported in the secretory and absorptive directions across Caco-2 cell monolayers. Caco-2 cells were
Exibir todos os documentos relacionados

Artigos

Drug transport assays in a 96-well system: reproducibility and correlation to human absorption

Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.

Caco-2 Intestinal Drug Transporter Models for More Predictive Drug Absorption

Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.

Conteúdo relacionado

Elementos essenciais para triagem de segurança, compostos-guia e alvos para descoberta de medicamentos

A triagem bem-sucedida de alvos de medicamentos e descoberta de compostos-guia requer reagentes e suprimentos testados, adequados para o processo e de alto rendimento. Encontre as ferramentas certas para seus processos de biotecnologia, farmacêuticos e CRO, para triagem de moléculas pequenas e biológicos, além de testes de imunogenicidade robustos para segurança terapêutica.

Lead Safety Screening for Drug Discovery

High-throughput reagents for drug target screening and immunogenicity testing.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica