MSP03

Membrane Scaffold Protein 1D1(-)

recombinant, expressed in E. coli, buffered aqueous solution

• Sinônimo(s): Membrane Scaffold Protein
• Código UNSPSC: 12352200
• NACRES: NA.26

Propriedades

• recombinante: expressed in E. coli
• Ensaio: ≥90% (SDS-GE)
• Formulário: buffered aqueous solution
• peso molecular: 22,043.9 Da
• solubilidade: water: soluble
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Research area: Cell structure. Membrane Scaffold Protein 1D1 (MSP1D1) is a variant of MSP1 where the first eleven amino acids in the helix 1 portion are deleted of the original MSP1 sequence. It consists of an N-terminal histidine-tagged protein with a tobacco etch virus (TEV) protease cleavage site that is present between the protein sequence and histidine-tag. MSP1D1(–)variant of MSP1D1 lacks the histidine-tag and the TEV protease-recognition sequence.

Aplicação

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

Ações bioquímicas/fisiológicas

Nano-disctechnology is an approach that allows aqueous soluble membrane proteins in anative-like bilayer environment that enables the membrane proteins to remainactive and stable. The concept of this technology is derived from high-densitylipoprotein (HDL) particles and apolipoprotein, their primary proteincomponent. The nano-disc is a non-covalent structure consisting of MSP that isgenetically engineered to mimic the activity of apolipoprotein A1 (Apo-A1) andphospholipid bilayer. In the presence of synthetic phospholipids, MSP1D1 canself-assemble into discoidal nanoparticlesknown as nano-discs. These nano-discs are suitable for studying membraneproteins and are applicable in the field of medicine and biotechnology.[1]

Informações legais

Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.

• 7,691,414 Membrane scaffold proteins
• 7,662,410 Membrane scaffold proteins and embedded membrane proteins
• 7,622,437 Tissue factor compositions and methods
• 7,592,008 Membrane scaffold proteins
• 7,575,763 Membrane scaffold proteins and tethered membrane proteins
• 7,083,958 Membrane scaffold proteins
• 7,048,949 Membrane scaffold proteins

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable