Based on previous results, a 20% difference in methylation is typically observable. It is recommended to conduct replicate experiments, assess the margin of error (technical and/or biological), and utilize a p-test or other statistical approach to evaluate its applicability in your specific case.
MDQ1
Kit de quantificação de DNA metilado Imprint®
To measure global DNA methylation shifts from as low as 10 ng DNA
Sinônimo(s):
DNA Methylation Quantification, DNA methylation shift
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
41116158
NACRES:
NA.54
Produtos recomendados
Descrição geral
A tecnologia de quantificação de DNA metilado Imprint™ fornece um método rápido e confiável para medir as alterações globais de metilação no DNA. O Kit de Quantificação de DNA metilado Imprint contém todos os reagentes necessários para detectar os níveis relativos de DNA metilado. Até 200 ng de DNA purificado estão ligados aos poços da tira de ensaio. O DNA metilado é detectado usando os anticorpos de captura e detecção e, em seguida, quantificado colorimetricamente. A quantidade de DNA metilado presente na amostra é proporcional à absorbância medida.
Aplicação
Características e benefícios
- Formato baseado em ELISA
- O procedimento é concluído em 4 etapas fáceis de seguir
- Todos os reagentes são fornecidos, inclusive o controle de DNA metilado
- O limite de detecção é de 5 ng de DNA totalmente metilado
- Eficaz a partir de apenas 10-200 ng de DNA inicial
- O procedimento é concluído em 3,5 horas
- O formato de 96 poços permite a opção de estudar amostras únicas ou estudos de alto rendimento
- Anticorpo e reagentes otimizados com alta especificidade até 5-mC; nenhuma reatividade cruzada com citosina não metilada
- Pode ser usado com células aderentes e em suspensão, tecidos, plasma e outros fluidos corporais, como saliva, urina e soro
Informações legais
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Componentes do kit também disponíveis separadamente
Nº do produto
Descrição
SDS
- M8570Methylated Control DNA, 50 ng/μLSDS
produto relacionado
Nº do produto
Descrição
Preços
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
Código de classe de armazenamento
8A - Combustible corrosive hazardous materials
Escolha uma das versões mais recentes:
Certificados de análise (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.
Se precisar de ajuda, entre em contato Atendimento ao cliente
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Mounira Krifa et al.
Journal of experimental & clinical cancer research : CR, 32, 30-30 (2013-05-22)
Several reports have described the potential effects of natural compounds as anti-cancer agents in vitro as well as in vivo. The aim of this study was to evaluate the anti-cancer effect of Limoniastrum guyonianum aqueous gall extract (G extract) and
Felicitas Thol et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 29(21), 2889-2896 (2011-06-15)
To study the incidence and prognostic impact of mutations in DNA methyltransferase 3A (DNMT3A) in patients with acute myeloid leukemia. A total of 489 patients with AML were examined for mutations in DNMT3A by direct sequencing. The prognostic impact of
Giulia F Del Gobbo et al.
Clinical epigenetics, 10, 34-34 (2018-03-23)
5,10-Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in one-carbon metabolism that ensures the availability of methyl groups for methylation reactions. Two single-nucleotide polymorphisms (SNPs) in the In 303 placentas screened for this study, we observed no significant association between the
Alok Mishra et al.
Methods in molecular biology (Clifton, N.J.), 863, 15-31 (2012-02-24)
Epigenetics is an emerging science that can help to explain carcinogenesis. The possibility that carcinogenesis may originate in a stem cell process was proposed recently. Stem cells are generated and contribute to tumor formation during the process of tumor development.
Roman Thaler et al.
The British journal of nutrition, 101(5), 743-749 (2008-08-08)
The impact of nutrition on the epigenetic machinery has increasingly attracted interest. The aim of the present study was to demonstrate the effects of various diets on methylation and gene expression. The antioxidative enzyme mitochondrial superoxide dismutase (MnSOD) was chosen
Artigos
Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.
Protocolos
The Imprint® Methylated DNA Quantification Kit provides a high-throughput, non-radioactive means of measuring global DNA methylation shifts.
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
-
Could you please provide the sensitivity of the kit and explain what differences in methylation status it can detect?
1 answer-
Helpful?
-
-
Are there any species restrictions for Product MDQ1, Imprint® Methylated DNA Quantification Kit?
1 answer-
The antibody works on 5-me-C, so any species with DNA that is methylated at the 5 position of cytidine residues will work.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What is the detection limit of Product MDQ1, Imprint® Methylated DNA Quantification Kit?
1 answer-
The detection limit of this kit is 5 nanograms of fully methylated DNA.
Helpful?
-
-
How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
Helpful?
-
-
When using Product MDQ1, Imprint® Methylated DNA Quantification Kit, can I dilute the entire antibody at once and save it for later?
1 answer-
No. A fresh dilution of the antibody should be prepared each time the kit is used. Diluting the antibody all at once will decrease its stability and it's effectiveness in the kit.
Helpful?
-
-
Is Product MDQ1, Imprint® Methylated DNA Quantification Kit, shipped in two parts?
1 answer-
Yes, Product MDQ1, Imprint® Methylated DNA Quantification Kit, is shipped in 2 parts. Part 1 contains, 2-8 °C components (W4267, D8568, C3493, D8818, D8693, S3447, and A4231) and Part 2 contains -20 °C components (M8570 and B6561).
Helpful?
-
-
When using Product MDQ1, Imprint® Methylated DNA Quantification Kit, do I need to use all the wells in the plate at once?
1 answer-
No, you can use as many wells as needed. You will want to ensure that all the wells that are not being used are covered well with Parafilm, Saran Wrap or some other plate sealer.
Helpful?
-
-
Can I use chloroform to extract my DNA before using Product MDQ1, Imprint® Methylated DNA Quantification Kit?
1 answer-
Choroform extraction should not interfere with the protocol as long as the DNA was not eluted with the chloroform.
Helpful?
-
Active Filters
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica
