MAK479

NADP/NADPH Assay Kit

sufficient for 100 colorimetric tests

• Código UNSPSC: 12161503
• NACRES: NA.84

Propriedades

• Nível de qualidade: 100
• método de detecção: colorimetric
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.

Características e benefícios

• The linear detection range of the kit is 0.1 µM to 10 µM NADP+ /NADPH
• Compatible with high-throughput handling systems.
• Convenient, the procedure involves adding a single working reagent, and reading the optical density at time zero and 30 minutes at room temperature.

Adequação

The kit is suitable for detection of NADP+ /NADPH concentrations and ratios in cell or tissue extracts.

Princípio

The NADP/NADPH Assay Kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+ /NADPH and is not interfered by NAD+ /NADH.

Informações de segurança

• Pictogramas: GHS02,GHS05,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H225,H290,H315,H319,H336
• Declarações de precaução: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 3 - Flammable liquids
• Classe de risco de água (WGK): WGK 2