MAK459

Detergent Assay Kit

Sufficient for 100 colorimetric tests

• Sinônimo(s): Detergent Detection Kit
• Código UNSPSC: 12161503
• NACRES: NA.84

Propriedades

• Nível de qualidade: 100
• condição de armazenamento: (No metal containers. Tightly closed.)
• entrada: protein; water; soil
• aplicação(ões): agriculture; environmental
• método de detecção: colorimetric
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Detergents play a significant role in increasing environmental pollution across various environments. This pollution originates from wastewater sources, such as residential areas where household detergents are used, agricultural runoff containing herbicides and insecticides, and certain industries.In biochemical research, detergents are commonly used for membrane protein separation. However, they can interfere with protein assays by affecting protein-protein interactions, enzymatic activities, or protein structure. Detecting and quantifying the presence of detergents is essential for accurate measurements in protein assays. Detergents used during protein extraction or purification processes may carry over into downstream applications, such as electrophoresis, chromatography, or mass spectrometry. This is crucial for maintaining assay accuracy, assessing protein functionality, ensuring compatibility with downstream applications, and preserving sample integrity.

Aplicação

The Detergent Assay Kit may be used for:

• Soil Testing
• Water Testing
• Detergent Detection in Biological Samples

Características e benefícios

Efficient Procedure: Streamline your workflow with a hassle-free 15-minute process, requiring only the addition of a single working reagent and a small 20 μL sample size, saving you valuable time and effort.

Linear Detection Range:

• Tween 80: Detect concentrations ranging from 0.012 to 4 mM
• Tween 20: Detect concentrations ranging from 0.06 to 5 mM
• Triton X-100: Detect concentrations ranging from 0.23 to 12 mM
• Brij L23/35: Detect concentrations ranging from 0.09 to 5 mM
• DTAC: Detect concentrations ranging from 0.08 to 2 mM
• SDS: Detect concentrations ranging from 10 to 25 mM

Adequação

The kit is suitable for detecting traces of detergent in purified protein samples and the determination of detergent in water and soil samples.

Princípio

Simple, direct and automation-ready procedures for measuring detergent concentration in biological samples are very desirable. The Detergent Assay Kit is designed to measure detergent directly without any pretreatment. Above the critical micellar concentration, detergent forms micelle in solution that trap the colorimetric dye. The intensity of the color is directly proportional to the detergent concentration in the sample.

Outras notas

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Informações de segurança

• Pictogramas: GHS05,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H290,H318,H410
• Declarações de precaução: P234 - P273 - P280 - P305 + P351 + P338 - P390 - P391
• Classificações de perigo: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1
• Código de classe de armazenamento: 8B - Non-combustible corrosive hazardous materials
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable