The supplier advises that they have not yet validated any lysis buffers for use with cell samples in conjunction with this kit. Additionally, they recommend using an untreated, clear, flat-bottom plate for colorimetric measurements. Therefore, it would be preferable to transfer the samples to such a plate for running the assay.
MAK317
Sorbitol Dehydrogenase Assay Kit
sufficient for 100 colorimetric tests
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R$ 2.914,00
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Produtos recomendados
método de detecção
colorimetric
doença(s) relevante(s)
endocrinological disorders, diabetes; gastrointestinal diseases
temperatura de armazenamento
−20°C
Categorias relacionadas
Descrição geral
Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage. Thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.[1][2]
Sorbitol Dehydrogenase (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage; thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy.
Características e benefícios
Compatible with high-throughput handling systems.
Adequação
Suitable for determining sorbitol dehydrogenase (SDH) activity in a number of biological samples such as plasma, serum, urine, tissue, and culture media.
Princípio
Sorbitol dehydrogenase assay is a non-radioactive, colorimetric assay. It is based on the reduction of the tetrazolium salt MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) in a NADH-coupled enzymatic reaction. The reduced form of MTT exhibits an absorption maximum at 565 nm and the increase in absorbance at 565 nm is directly proportional to the enzyme activity. This assay is based on a kinetic reaction. Sorbitol dehydrogenase (SDH) activity can be determined in a number of biological samples (e.g., plasma, serum, urine, tissue, and culture media).
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
Órgãos-alvo
Respiratory system
Código de classe de armazenamento
3 - Flammable liquids
Ponto de fulgor (°F)
75.2 °F - closed cup
Ponto de fulgor (°C)
24 °C - closed cup
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What are the recommended methods for homogenizing or lysing cells in a 96-well plate for the MAK317 Sorbitol Dehydrogenase assay kit? The manual advises against using proteolytic enzymes and suggests using a rubber policeman, which is not suitable for a 96-well plate. Are there any recommendations for a mild lysing buffer with minimal impact on the assay outcome? Additionally, is it possible and recommended to run the assay in the same 96-well plate, or is transferring the samples to a new plate necessary?
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