MAK159
Mitochondrial Membrane Potential Kit
sufficient for 500 fluorometric tests (microplate readers)
Sinônimo(s):
JC-10 Assay, JC-10 Mitochondrial Membrane Potential Assay
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12161503
NACRES:
NA.25
Produtos recomendados
uso
sufficient for 500 fluorometric tests (microplate readers)
método de detecção
fluorometric
doença(s) relevante(s)
cancer
temperatura de armazenamento
−20°C
Descrição geral
Mitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
Aplicação
Mitochondrial Membrane Potential Kit has been used to measure mitochondrial membrane potential.
Adequação
This kit is suitable for the detection of Mitochondrial Membrane Potential in mammalian cells and for screening apoptosis inhibitors and activators using microplate readers.
Princípio
This kit utilizes JC-10, a superior alternative to JC-1, for determining the loss of the MMP in cells. Although JC-1 is widely used in many labs, its poor water solubility often results in precipitation in aqueous buffers when used at higher concentrations. At higher concentrations, JC-10 exhibits greater aqueous solubility than JC-1. Similar to JC-1, JC-10 is a cationic, lipophilic dye that is concentrated and forms reversible red-fluorescent JC-10 aggregates (λex = 540/λem = 590 nm) in the mitochondria of cells with a polarized mitochondrial membrane. In apoptotic cells, MMP collapse results in the failure to retain JC-10 in the mitochondria and a return of the dye to its monomeric, green fluorescent form (λex = 490/λem = 525 nm). This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Na Yang et al.
Biochemical and biophysical research communications, 517(1), 111-117 (2019-07-16)
Doxorubicin (DOX) is a potent anti-neoplastic agent with cumulative cardiotoxicity. DOX-induced cardiotoxicity has been shown to depend on the different dosing times. However, the basis for determining the dosing time to minimize DOX-induced cardiotoxicity and the underlying mechanisms remain incompletely
Hemlata et al.
ACS omega, 6(15), 10383-10395 (2021-06-01)
In recent decades, biodegradable polymeric nanoparticles have been used as a nanocarrier for the delivery of anticancer drugs. In the present study, we synthesize bovine serum albumin (BSA) nanospheres and evaluate their ability to incorporate a plant extract with anticancer
Giulia Allavena et al.
Cell death & disease, 9(7), 780-780 (2018-07-15)
The cellular recycling pathway of autophagy plays a fundamental role in adaptive responses to nutrient deprivation and other forms of stress under physiological and pathological conditions. However, autophagy can also be a double-edge sword during certain bacterial infections (such as
The mitochondrial membrane potential (deltapsi(m)) in apoptosis.
Jennifer D L, et al.
Apoptosis, 8, 116-128 (2003)
Stearic acid supplementation in high protein to carbohydrate (P: C) ratio diet improves physiological and mitochondrial functions of Drosophila melanogaster parkin null mutants.
Bajracharya R, et al.
The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences, glx246-glx246 (2017)
Artigos
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica