Product No. MAK107 comes with a "Substrate Reagent" component which is used as the substrate in this assay.
MAK107
LCAT Activity Assay Kit
Supplied by Roar Biomedical, Inc.
Sinônimo(s):
Lecithin:cholesterol acyltransferase activity assay kit
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About This Item
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
uso
sufficient for 100 fluorometric tests
aplicação(ões)
pharmaceutical
método de detecção
fluorometric
doença(s) relevante(s)
cardiovascular diseases
temperatura de armazenamento
−20°C
Informações sobre genes
human ... LCAT(3931)
Descrição geral
The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol resulting in the formation cholesteryl ester. This enzymatic activity occurs on the surface of high density lipoprotein (HDL). The cholesteryl esters formed by LCAT may be packed into the core of HDL.
Aplicação
Applications for this method include high-throughput screening, mechanism of action studies and structure-activity relationship (SAR) work.
Características e benefícios
Compatible with high-throughput handling systems.
Adequação
Suitable for measuring phospholipase activity of LCAT in plasma or serum.
Princípio
The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of (λEx=390 nm/λEm=470 nm) LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman′s reagent) LCAT inhibitor.
Somente componentes do kit
Nº do produto
Descrição
- Substrate Reagent .1 mL
- READ Reagent 30 mL
- LCAT Assay Buffer 20 mL
produto relacionado
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
Código de classe de armazenamento
3 - Flammable liquids
Ponto de fulgor (°F)
49.5 °F
Ponto de fulgor (°C)
9.7 °C
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What substrate should be used to create the standard curve for this assay?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Are products MAK107 and MAK108 compatible with mouse serum?
1 answer-
Yes, but please be aware of possible sample hemolysis. Depending on the method of mouse sample collection, hemolysis may be common, which can interfere with fluorescence emission via color quenching.
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