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M7383

Sigma-Aldrich

Malic Dehydrogenase from porcine heart

≥400 units/mg protein (biuret), ammonium sulfate suspension

Sinônimo(s):

L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase

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About This Item

Número da licença da enzima:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

forma

ammonium sulfate suspension

atividade específica

≥400 units/mg protein (biuret)

atividade externa

Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%

temperatura de armazenamento

2-8°C

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Descrição geral

Cytoplasmic Isozyme

Aplicação

Malic dehydrogenase has been used in a study to assess the use of a long-wavelength homogeneous enzyme immunoassay for the determination of amikacin in water samples. It has also been used in a study to investigate the lipid metabolism in a liver exposed to cadmium.

Ações bioquímicas/fisiológicas

The surfactant cetyl (hexadecyl) trimethylammonium bromide inhibits the cytoplasmic isoform of porcine heart malate dehydrogenase but has no effect on the mitochondrial form. SDS however; inhibits both isoforms.

Definição da unidade

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

forma física

Suspension in 3.2 M (NH4)2SO4, 0.1 M KH2PO4, pH 7.0

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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