L9639

Ricin B chain from Ricinus communis (castor bean)

buffered aqueous solution

• Número CAS: 96638-29-8
• Número MDL: MFCD00166673
• Código UNSPSC: 12352200

Propriedades

• forma: buffered aqueous solution
• temperatura de armazenamento: 2-8°C

Descrição

Ações bioquímicas/fisiológicas

Ricin B chain has an affinity for galactose and saccharides containing nonreducing terminal β-galactosyl units. This affinity allows the B chain to bind to cell surface glycoconjugates. In the intact ricin toxin, the surface binding by the B chain allows the A chain to enter into the cell. The saccharide binding capability of ricin B chain is independent of the presence of the A chain.

Outras notas

Ricinus communis toxin (also named RCA₆₀, RCA_II, Ricin D, or RCL III) is a highly toxic protein, mol. wt. ~60 kDa, composed of two chains, A and B, connected by a single disulfide bridge.

Qualidade

Electrophoretically pure (SDS-PAGE)

forma física

Solution in 10 mM phosphate, pH 6.5, containing 0.15 M NaCl, 10 mM galactose, 0.5 mM dithioerythritol and 0.02% sodium azide

Nota de preparo

Highly purified by affinity chromatography

Nota de análise

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg protein per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable