L2897

LB Broth with agar (Lennox)

Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

• Sinônimo(s): Lennox broth, Luria Bertani Agar
• Código UNSPSC: 41106200
• NACRES: NA.85

Propriedades

• grau: for molecular biology
• Nível de qualidade: 200
• esterilidade: non-sterile
• forma: powder
• composição: Agar, 15 g/L ; NaCl, 5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• técnica(s): microbiological culture: suitable
• aplicação(ões): food and beverages; microbiology
• temperatura de armazenamento: room temp
• adequação: nonselective for Escherichia coli; nonselective for coliforms

Descrição

Descrição geral

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Aplicação

LB Broth with agar (Lennox) has been used for:

• the transformation of competent E. coli cells
• to cultivate Ecc-15 bacteria
• embedding scaffold containing tissue derived from induced pluripotent stem cells (iPSCs)
• transmission electron microscopy (TEM) and immunohistochemistry (IHC)

It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

Características e benefícios

Lennox LB powder with agar provides:

• A budget-friendly alternative to pre-poured plates
• Easy scale-up using larger package sizes
• Standard formulation

Nota de preparo

1. Suspend 35 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving.

Informações legais

Zeocin is a registered trademark of Cayla Sarl

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)