I8260

IgM from human serum

reagent grade, ~95% (HPLC), buffered aqueous solution

• Sinônimo(s): Human IgM
• Número MDL: MFCD00163928
• Código UNSPSC: 12352203
• NACRES: NA.46

Propriedades

• conjugado: unconjugated
• grau: reagent grade
• Ensaio: ~95% (HPLC)
• Formulário: buffered aqueous solution
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified

Descrição

Descrição geral

There are five immunoglobulin classes in humans. Out of these, immunoglobulin M (IgM) is a high molecular weight protein that has five or six subunits. IgM monomers are made of two heavy chains and two light chains connected by a disulfide bond. Human serum shows low concentration of IgM monomers. It has a high carbohydrate content of about 12%. It is the first immunoglobulin produced by neonates.[1]
IgM antibodies are present as pentamers in the serum and are produced in response to antigens.[2]
IgM from human serum is purified from normal human serum by precipitation and gel filtration techniques. The immunoglobulin is determined to be atleast 95% pure by HPLC procedures.

Aplicação

Purified human IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. IgM from human serum was used as standard in ELISA (100 μg/ml)[3] and dot blot assay.[4] Antibody concentration range of 200 μg/ml to 1 mg/ml was used in IgM binding assays.[5][6]
IgM from human serum has been used

• in ELISA inhibition assay[7]
• in immunoblotting[7]
• as MW (molecular weight) standards[8]
• in IgM binding assay[9]
• to confirm the depletion of immunoglobulins[10]

Ações bioquímicas/fisiológicas

Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions.[1]

forma física

Solution in 0.05 M Tris-HCl, 0.2 M sodium chloride, pH 8.0, containing 15 mM sodium azide.

Armazenamento e estabilidade

Store at 2-8 °C. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter