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HSANGERV

Sigma-Aldrich

Sanger Arrayed Whole Genome Lentiviral CRISPR Library

Human, Virus Format

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About This Item

Código UNSPSC:
41105904

Nível de qualidade

200

embalagem

pkg of 10 μL (384-well plate)

concentração

1x106  VP/ml (via p24 assay)

aplicação(ões)

CRISPR

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

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Categorias relacionadas

Descrição geral

Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.

Aplicação

Functional Genomics/Screening /Target Validation

Características e benefícios

  • Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
  • Simplify the workflow with puromycin selection
  • Illuminate CRISPR-expressing cells with BFP

Additional Features
  • Better, not bigger: Two optimized clones per gene reduces the time, cost, and scale of screening experiments
  • Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
  • Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

Embalagem

384-well plates

Componentes

Lentivirus Transduction Particles of gRNA-only lenti-based vector. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones.


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forma física

10 μl of lentivirus at ≥106 particles/ml in 102×384-well plates

Outras notas

This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

Produtos recomendados

Additional libraries are available:
Human, Glycerol Format
Mouse, Glycerol Format
Mouse, Virus Format

Informações legais

CRISPR Use License Agreement

Lentiviral License Agreement

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.
Metzakopian, E. et al.
Scientific Reports, 7 (2017)

Artigos

The Researcher’s Guide to Designing CRISPR and RNAi Screening Experiments

This screening guide covers how to choose a cell line, a screening library, and experimental conditions as well as tips for designing and performing your experiment.

Sanger Whole Genome CRISPR Libraries

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

Protocolos

Sanger Sequencing Steps & Method

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

Etapas e método de sequenciamento de Sanger

Saiba mais sobre as etapas do sequenciamento Sanger ou o método de terminação de cadeia e como funciona o sequenciamento de DNA e como ler os resultados do sequenciamento de Sanger com precisão para sua pesquisa.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

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