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GE80-6483-56

2-D Quant Kit

Cytiva 80-6483-56

Sinônimo(s):

2D Quantification Kit, Protein Quant Kit

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About This Item

Código UNSPSC:
41105323
NACRES:
NA.25

Características

wetted part: no

fabricante/nome comercial

Cytiva 80-6483-56

embalagem

pkg of 500 assays

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.

Common spectrophotometric methods of quantitating protein rely either on Coomassie dye binding or protein-catalyzed reduction of cupric (Cu2+) ion to cuprous (Cu+) ion. Dye-binding assays cannot be used in the presence of any reagent that also binds the dye. This includes carrier ampholytes and detergents such as PlusOne CHAPS, SDS, and Triton X-100. Assays that depend on the reduction of cupric ion cannot be used in the presence of reductants such as DTT, or in the presence of reagents that form complexes with cupric ion, such as thiourea or EDTA.

The 2-D Quant Kit procedure works by quantitatively precipitating proteins while leaving interfering substances behind. The assay is based on the specific binding of copper ions to protein. Precipitated proteins are resuspended in a copper-containing solution and unbound copper is measured with a colorimetric agent. The absorbance at 480 nm is inversely related to the protein concentration. The assay has a linear response to protein in the range of 0 to 50 μg and recommended sample volume is 1 to 50 μ.

Características e benefícios

  • Accurately determine protein concentration in the presence of 2% SDS, 1% DTT, 8 M urea, 2 M thiourea, 4% CHAPS, 2% Pharmalyte, and 2% IPG Buffer.
  • Quantitatively precipitates proteins while leaving interfering substances behind.
  • Linear response in the range of 0 to 50 μg protein, with recommended sample volumes of 1 to 50 μ.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Pictogramas

Environment
GHS09

Palavra indicadora

Danger

Frases de perigo

H314,H335,H411

Código de classe de armazenamento

8B - Non-combustible corrosive hazardous materials

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Milan O Urban et al.
Frontiers in plant science, 12, 628167-628167 (2021-06-29)
The present study aims to investigate the response of rapeseed microspore-derived embryos (MDE) to osmotic stress at the proteome level. The PEG-induced osmotic stress was studied in the cotyledonary stage of MDE of two genotypes: Cadeli (D) and Viking (V)

Protocolos

Protein Determination using 2-D Quant Kit

2-D Quant Kit from Cytiva is designed to accurately determine protein concentrations in samples for electrophoresis.

Desalting Samples with Mini Dialysis Kit

Mini Dialysis Kit from Cytiva minimizes sample handling and loss, reducing streaking on 2-D gels from contaminants.

Cleaning Up Samples using 2-D Clean-Up Kit

2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.

Western Blotting Sample Preparation

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

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