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GE17-5279-02

Glutathione Seph High Performance

Name: Glutathione Seph High Performance
Brand: SIGMA

Cytiva 17-5279-02, pack of 100 mL

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About This Item

Código UNSPSC:

23151817

NACRES:

NA.56

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Glutathione Sepharose^™ 4B

Supelco

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Millipore

G4510

Glutathione−Agarose

Sigma-Aldrich

G5080

Sephadex^® G-50

ligand

glutathione

embalagem

pack of 100 mL

fabricante/nome comercial

Cytiva 17-5279-02

Matriz

highly cross-linked 6% agarose

Tamanho médio da partícula

34 μm

cleaning

3-12

Faixa de trabalho

3-12

capacidade

>7 mg/mL binding capacity(medium)

Categorias relacionadas

Cytiva™

Descrição geral

Glutathione Sepharose^™ High Performance is an affinity medium for high resolution purification of Glutathione S transferase (GST) tagged proteins.

Aplicação

Glutathione Sepharose^™ High Performance is used for the purification of GST-tagged Proteins from bacterial lysates and other S-transferases or glutathione-dependent Proteins. GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function.

Características e benefícios

34 μm bead size for improved efficiency and resolution
Fast, one-step purification of GST-tagged Proteins and other glutathione S-trasferase or glutathione-dependent Proteins
Mild conditions during the purification process preserves Protein antigenicity and function

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a trademark of Cytiva

Pictogramas

GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Declarações de precaução

P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501

Código de classe de armazenamento

3 - Flammable liquids

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Certificados de análise (COA)

Lot/Batch Number

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Artigos

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

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Protocolos

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

GSTrap™ Columns for Purification

GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.

Characteristics of Glutathione Sepharose® Products

Glutathione Sepharose® High Performance is recommended for high-resolution puriﬁcation of GST-tagged proteins, providing sharp peaks and concentrated eluent.