GE17-5154-01

HiTrap^® DEAE Fast Flow

Cytiva 17-5154-01, pack of 5 × 5 mL

• Sinônimo(s): DEAE HiTrap Column, Fast Flow DEAE Column
• Código UNSPSC: 23151816
• NACRES: NA.56

Propriedades

• ligand: diethylaminoethyl
• descrição: Ion Exchanger Type (value)
• embalagem: pack of 5 × 5 mL
• fabricante/nome comercial: Cytiva 17-5154-01
• Parâmetros: 42 psi
• tamanho do leito: 16 mm × 25 mm
• volume do leito: 5 mL
• D.I. da coluna: 16 mm
• matriz: 6% cross-linked agarose
• tamanho de partícula: 45-165 μm
• diâmetro médio: 90 μm
• cleaning: 2-14
• working range: 2-12
• adequação: suitable for bioprocess medium

Descrição

Descrição geral

HiTrap^® DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Q, SP, DEAE, and CM Sepharose^™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose^™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose^™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose^™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached.

Características e benefícios

• Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
• The industry standard for ion exchange chromatography.
• High flow rates and good scale-up potential.
• Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
• Predictable scale-up

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Outras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informações legais

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Informações de segurança

• Pictogramas: GHS02
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de classe de armazenamento: 3 - Flammable liquids