GE17-5055-01
HiTrap® DEAE Fast Flow
Cytiva 17-5055-01, pack of 5 × 1 mL
Sinônimo(s):
DEAE resin, Ion-exchange chromatography resin
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About This Item
Código UNSPSC:
23151816
NACRES:
NA.56
ligand
diethylaminoethyl
descrição
Ion Exchanger Type (value)
embalagem
pack of 5 × 1 mL
fabricante/nome comercial
Cytiva 17-5055-01
Parâmetros
42 psi
tamanho do leito
7 mm × 25 mm
volume do leito
1 mL
D.I. da coluna
7 mm
matriz
6% cross-linked agarose
tamanho de partícula
45-165 μm
diâmetro médio
90 μm
cleaning
2-14
working range
2-12
adequação
suitable for bioprocess medium
Categorias relacionadas
Descrição geral
HiTrap® DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
Aplicação
Q, SP, DEAE, and CM Sepharose™ Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.
The active end of the charged group is the same for DEAE Sepharose Fast FLow and ANX Sepharose™ Fast FLow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast FLow has a diethylaminoethyl-group bound to the Agarose whilst ANX Sepharose™ 4 Fast FLow (high sub) has a diethylaminopropyl group attached.
The active end of the charged group is the same for DEAE Sepharose Fast FLow and ANX Sepharose™ Fast FLow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast FLow has a diethylaminoethyl-group bound to the Agarose whilst ANX Sepharose™ 4 Fast FLow (high sub) has a diethylaminopropyl group attached.
Características e benefícios
- Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
- The industry standard for ion exchange chromatography.
- High fLow rates and good scale-up potential.
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
- Predictable scale-up
Armazenamento e estabilidade
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Nota de análise
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Informações legais
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Código de classe de armazenamento
3 - Flammable liquids
Certificados de análise (COA)
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