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G3293

Supelco

Reagente de ensaio de glicose

for use with enzymatic assay kits GAHK20, SCA20, FA20, SA20

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About This Item

Código UNSPSC:
12164500
NACRES:
NA.84
Preço e disponibilidade não estão disponíveis no momento.

classe(s) química(is) do analito

sugars (glucose)

técnica(s)

photometry: suitable

Impurezas

≤0.005% Creatine phosphokinase, glutathione reductase, myokinase, NADH-oxidase,NADPH oxidase, phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase
<0.01% ATP-ase, myokinase

aplicação(ões)

food and beverages
general analytical

temperatura de armazenamento

2-8°C

Categorias relacionadas

Especificidade

<< 0,05% de Atividade resultante de reatividade cruzada com os seguintes substratos: frutose, dextrana, sedo-heptulose, arabinose, galactose, xilose, trealose, manose, sorbitol, +/- fucose ou ribose.

Aplicação

Para a determinação quantitativa da glicose em alimentos e outros materiais utilizando o ensaio enzimático da hexoquinase-glicose 6-fosfato desidrogenase.
Reconstituir com água desionizada utilizando o volume indicado com base no tamanho da embalagem (20 ou 50 ml).

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Timestrip Plus™ 8 °C

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
0000390450
0000390450
0000409790
0000409790
0000389932

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Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.

Pesquise por COA

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Daniel S Straus
Molecular cancer, 12, 78-78 (2013-07-20)
Inflammation is a well-known etiological factor for colorectal cancer, but mechanisms underlying the linkage between inflammation and cancer are incompletely understood. We hypothesized that two pro-inflammatory cytokines, TNFα and IL-17, might play a role in promoting colorectal carcinogenesis. Aerobic glycolysis
Anne Riemann et al.
PloS one, 6(7), e22445-e22445 (2011-08-06)
Tumor micromilieu often shows pronounced acidosis forcing cells to adapt their phenotype towards enhanced tumorigenesis induced by altered cellular signalling and transcriptional regulation. In the presents study mechanisms and potential consequences of the crosstalk between extra- and intracellular pH (pH(e)
Michele Bertazzini et al.
Frontiers in plant science, 7, 288-288 (2016-03-26)
Numerous angiosperms rely on pollinators to ensure efficient flower fertilization, offering a reward consisting of nourishing nectars produced by specialized floral cells, known as nectaries. Nectar components are believed to derive from phloem sap that is enzymatically processed and transformed
Lara Kern et al.
Cell and tissue research, 366(3), 651-665 (2016-08-25)
Autophagy is a lysosomal degradation process involved in the turnover of organelles or other cell constituents, in providing sources for energy production under starving conditions and in cell metabolism. A key protein in the macroautophagic machinery is the autophagy-related protein
Chao Zhang et al.
Ecotoxicology (London, England), 29(2), 175-184 (2020-01-16)
A key challenge for ecological risk assessment of contaminants under global warming is to predict effects at higher levels of biological organisation. One approach to reach this goal is to study how contaminants and warming cause changes in body stoichiometry
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Protocolos

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

Questions

1–8 of 8 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. For how many tests this reagent will be sufficient?

    1 answer

    1. This product is available in a 20 mL or 50 mL package size. The entire vial should be reconstituted in DI water as indicated by the package size. Each test requires 1 mL of this reagent. The 20 mL package size is sufficient to perform 20 test and the 50 mL pack size is sufficient to perform 50 tests.

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  4. How much of the initial must be reconstituted in 20 ml of DI water?

    1 answer

    1. The entire vial should be reconstituted to the volume of the package size. The G3293-20 mL with 20 mL of DI water and the G3293-50 mL with 50 mL of DI water.

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  5. Is magnesium required for Product G3293, Glucose Assay Reagent?

    1 answer

    1. Magnesium, an activator of hexokinase, is included in the assay reagent. There is no need to add a magnesium salt to the reagent before use.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  7. Is it possible to adapt Product G3293, Glucose Assay Reagent, for use with a 96 well plate reader?

    1 answer

    1. In principle, it should be possible to adapt assay procedures using this reagent to 96-well plate readers. Volumes of reagent, water, standard, and unknown should be reduced to fit the capacity of the test wells, and kept in the same proportions as those used with a spectrophotometer. The volume reduction will likely be 1:4 or 1:5. With the shorter "pathlength" of a multiwell plate, the sensitivity of the assay would likely decrease.

      Helpful?

  8. What is the detection limit of Product G3293, Glucose Assay Reagent?

    1 answer

    1. This reagent should be able to detect glucose down to approximately 0.01 mg/mL using a spectrophotometer with a 1 cm path length.

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