F2922
Monoclonal Anti-FLAG® BioM5−Biotin antibody produced in mouse
clone M5, purified immunoglobulin, buffered aqueous solution
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Número MDL:
Código UNSPSC:
12352203
Produtos recomendados
fonte biológica
mouse
conjugado
biotin conjugate
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
M5, monoclonal
forma
buffered aqueous solution
técnica(s)
western blot (chemiluminescent): 2 μg/mL
Isotipo
IgG1
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Descrição geral
Anti-FLAG® BioM5 monoclonal antibody is a purified murine IgG1 monoclonal antibody that is covalently attached by hydrazide linkage. It can be detected by avidin or streptadivin conjugates.
Aplicação
Anti-FLAG® BioM5 monoclonal antibody is useful for Western blotting, microscopy applications and formation of avidin-biotin complexes (ABC) in mammalian and Drosophila cells. Anti-FLAG® BioM5 antibody in combination with an avidin or streptavidin conjugate is the preferred anti-FLAG® antibody for detection of FLAG fusion proteins expressed in mouse cells.
The product binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. It is useful for detecting cytoplasmically expressed Met-FLAG® fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
It can be used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Browse additional application references in our FLAG® Literature portal.
The product binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. It is useful for detecting cytoplasmically expressed Met-FLAG® fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
It can be used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Browse additional application references in our FLAG® Literature portal.
Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
Características e benefícios
ANTI-FLAG M5 − Biotin conjugate has a high affinity for N-terminal Met-FLAG fusion proteins.
forma física
Solution in 10 mM sodium phosphate, pH 7.4, containing 150 mM NaCl and 0.02% sodium azide
Informações legais
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Jin-Ho Koh et al.
Cell metabolism, 25(5), 1176-1185 (2017-05-04)
The objective of this study was to evaluate the specific mechanism(s) by which PPARβ regulates mitochondrial content in skeletal muscle. We discovered that PPARβ increases PGC-1α by protecting it from degradation by binding to PGC-1α and limiting ubiquitination. PPARβ also
Xinna Zhang et al.
The EMBO journal, 30(11), 2177-2189 (2011-04-28)
Tumour suppressor p53 levels in the cell are tightly regulated by controlled degradation through ubiquitin ligases including Mdm2, COP1, Pirh2, and ARF-BP1. The ubiquitination process is reversible via deubiquitinating enzymes, such as ubiquitin-specific peptidases (USPs). In this study, we identified
Peipei Wang et al.
The Journal of biological chemistry, 299(8), 105055-105055 (2023-07-17)
Post-translational modifications including protein ubiquitination regulate a plethora of cellular processes in distinct manners. RNA N6-methyladenosine is the most abundant post-transcriptional modification on mammalian mRNAs and plays important roles in various physiological and pathological conditions including hematologic malignancies. We previously
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica