The promoter used in this product is CVM which is designed for use with Mammalian systems. This product has not been tested outside of Mammalian systems.
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About This Item
Código UNSPSC:
41106609
NACRES:
NA.51
Produtos recomendados
recombinante
expressed in E. coli
Nível de qualidade
embalagem
vial of 50 μL
concentração
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Categorias relacionadas
Descrição geral
This product is an expression plasmid that utilizes the CMV promoter for strong transient expression of eSpCas9 and GFP linked by a 2A peptide (CMV-eSpCas9-2A-GFP) allowing for easy visualization of successful transfection. The eSpCas9 expression plasmid is one part of a two part CRISPR system with individual eSpCas9 and gRNA expression vectors.
To order gRNA in any format click here
To order gRNA in any format click here
Aplicação
Functional Genomics/Target Validation
- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags, or reporters integrated into endogenous genes
Características e benefícios
- Enhanced specificity compared to wild type Cas9
- Highly Active
- Ready to use purified plasmid DNA
Princípio
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB. Newly engineered eSpCas9 enables the efficient targeted gene editing of established CRISPR systems with the benefit of reduced off-target effects. Point mutations in the chromosome-binding motif of SpCas9, as described by Slaymaker, et al., provide higher on-target fidelity without loss of cleavage efficiency.[1]
Informações legais
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Certificados de análise (COA)
Lot/Batch Number
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Rationally engineered Cas9 nucleases with improved specificity.
Slaymaker, I.M., et al.
Science, 351, 84-88 (2015)
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Is this plasmid suitable for bacteria amplification and metazoan GFP expression?
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