EPI010
Sirtuin 2 (SIRT2) Inhibitor Screening Assay Kit
100 assays in 96 well plates
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.41
Produtos recomendados
uso
100 assays in 96 well plates
condição de armazenamento
protect from light
nº de adesão NCBI
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Informações sobre genes
human ... SIRT2(22933)
mouse ... SIRT2(64383)
Descrição geral
Sirtuin 2 (SIRT2) is a cytoplasmic protein and is part of the sirtuin family of proteins.[1] Sirtuin family contains a sirtuin core domain and isgrouped into four classes with SIRT2 being a member of class I. SIRT2 colocalizes with microtubules.[2][3] The gene encoding this protein is localized on human chromosome 19q13.2.[4]
With Sigma′s Sirtuin Inhibitor Screening Kit, Sirtuin deacetylates the Substrate, and then a Developer cleaves the deacetylated substrate to release a fluorescent group, the latter of which can be detected at Ex/Em = 395/541 nm. In the presence of a SIRT inhibitor, the deacetylation will be impeded, preventing substrate cleavage and release of the fluorescent group. The kit provides a rapid, simple, sensitive, and reliable test, which is suitable for both low and high-throughput screening of SIRT2 inhibitors. A positive control inhibitor, Nicotinamide, is included to compare with the efficacy of the test inhibitors.
With Sigma′s Sirtuin Inhibitor Screening Kit, Sirtuin deacetylates the Substrate, and then a Developer cleaves the deacetylated substrate to release a fluorescent group, the latter of which can be detected at Ex/Em = 395/541 nm. In the presence of a SIRT inhibitor, the deacetylation will be impeded, preventing substrate cleavage and release of the fluorescent group. The kit provides a rapid, simple, sensitive, and reliable test, which is suitable for both low and high-throughput screening of SIRT2 inhibitors. A positive control inhibitor, Nicotinamide, is included to compare with the efficacy of the test inhibitors.
Aplicação
Sirtuin 2 (SIRT2) inhibitor screening assay kit has been used for acetyl peptide deacetylase assay.[5]
Ações bioquímicas/fisiológicas
Sirtuin 2 (SIRT2) maintains the integrity of the genome.[1] Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington′s disease. Huntington′s disease is characterized by increased sterol synthesis in neuronal cells and this process is reversed by SIRT2 inhibition.[6] SIRT2 can deacetylate lys40 of α-tubulin both in vitro and in vivo. Knockdown of SIRT2 by small interfering RNA (siRNA) leads to tubulin hyperacetylation.[3]
Características e benefícios
- Simple, sensitive, and reliable assay
- Species reactivity: mammalian
- Utilizes fluorometric methods
- Suitable for individual tests or high throughput assays
- Convenient 96-well microplate format
- Screening and characterization of Sirtuin inhibitors
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Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - STOT SE 3
Órgãos-alvo
Respiratory system
Código de classe de armazenamento
10 - Combustible liquids
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The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase.
North BJ
Molecular and Cellular Biochemistry, 11(2) (2003)
Damir Janigro
The Cell Cycle in the Central Nervous System (2008)
Proteomics-based identification of differentially expressed genes in human gliomas: down-regulation of SIRT2 gene
Masaharu Hiratsuka
Biochemical and Biophysical Research Communications (2003)
Brian P Weiser et al.
The Journal of biological chemistry, 290(13), 8559-8568 (2015-02-11)
meta-Azi-propofol (AziPm) is a photoactive analog of the general anesthetic propofol. We photolabeled a myelin-enriched fraction from rat brain with [(3)H]AziPm and identified the sirtuin deacetylase SIRT2 as a target of the anesthetic. AziPm photolabeled three SIRT2 residues (Tyr(139), Phe(190)
Propofol Inhibits SIRT2 Deacetylase through a Conformation-specific, Allosteric Site*
Brian P
The Journal of Biological Chemistry (2015)
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