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Documentos Principais

D8899

Sigma-Aldrich

Deoxyribonucleic acid, single stranded from calf thymus

lyophilized powder

Sinônimo(s):

DNA single stranded

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About This Item

Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
41106310
eCl@ss:
32160414
NACRES:
NA.52

grau

for molecular biology

Nível de qualidade

Formulário

lyophilized powder

peso molecular

~50 kb

composição

ssDNA, ≥65%

temperatura de armazenamento

−20°C

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Descrição geral

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Aplicação

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Características e benefícios

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Quantidade

One mg DNA is approx. 25 A260 units.

Nota de preparo

Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

Reconstituição

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves


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H Kamei
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