D8281

Anti-Desmin antibody produced in rabbit

whole antiserum

• Sinônimo(s): Anti-Desmin, Desmin Detection Antibody, Rabbit Anti-Desmin
• Número MDL: MFCD00162258
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: whole antiserum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• contém: 15 mM sodium azide
• reatividade de espécies: chicken, bovine, human, mouse
• validação aprimorada: independent; Learn more about Antibody Enhanced Validation
• técnica(s): indirect immunofluorescence: 1:20 using cultured cells; indirect immunofluorescence: 1:20 using human skeletal muscle sections; western blot: 1:100 using animal or human skeletal muscle.
• nº de adesão UniProt: P17661
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... DES(1674) ; mouse ... Des(13346)

Descrição

Descrição geral

Desmin is an intermediate filament protein found mainly in skeletal, cardiac, and smooth muscle cells. It acts as a marker for tumor origin. It also facilitates mechanical support for muscles, mitochondrial localization, myofibrillogenesis, gene expression regulation, and intracellular signalling. Anti-desmin antibody can be used for immunocytochemical localization of intermediate filaments in all types of muscle cells. It can also be used to stain a wide desmin band of 50,000 to 55,000 molecular weight using immunoblotting assays. Rabbit anti-desmin antibody reacts specifically with desmin in bovine, mouse, chicken and human.

Desmin is mapped to human chromosome 2q35. The desmin protein comprises of a head domain, four α-helical segments, three non-helical rod segments and a tail domain. It exists as dimer and tetramer assemblies. In smooth and striated muscles, desmin is localized in the periphery of Z-disk.

Imunogênio

desmin from chicken gizzard

Aplicação

Anti-Desmin antibody produced in rabbit has been used in:

• immunohistochemistry of zebrafish intestines sections
• immunodetection in fish embryo samples
• immunolabeling of myofibers

Anti-desmin antibody can be used in immunocytochemistry and western immunoblotting. It can also be used in indirect immunofluorescence.

Ações bioquímicas/fisiológicas

Desmin plays a crucial role in providing mechanical strength to muscle tissues. Mutations in desmin gene contributes to imbalance in skeletal proteins, resulting in desminopathies. Missense mutations in desmin gene leads to hypertrophic cardiomyopathy and systemic myopathy. Mutations in desmin gene impairs the stability of intermediary filaments in cardiomyocytes, contributing to pathophysiology of cardiac diseases.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable