D8168

Anti-Dystrophin Antibody

mouse monoclonal, MANDYS8

• Sinônimo(s): Dystrophin Antibody, Dystrophin Antibody - Monoclonal Anti-Dystrophin antibody produced in mouse
• Número MDL: MFCD00164586
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• Nome do produto: Monoclonal Anti-Dystrophin antibody produced in mouse, clone MANDYS8, ascites fluid
• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: ascites fluid
• tipo de produto de anticorpo: primary antibodies
• clone: MANDYS8, monoclonal
• reatividade de espécies: chicken, rat, human, pig, rabbit, mouse
• técnica(s): indirect ELISA: suitable; indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections.; microarray: suitable; western blot: suitable
• Isotipo: IgG2b
• nº de adesão UniProt: P11532
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... DMD(1756) ; mouse ... Dmd(13405) ; rat ... Dmd(24907)

Descrição

Descrição geral

Monoclonal Anti-Dystrophin (mouse IgG2b isotype) is derived from the MANDYS8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Dystrophin is a structural protein on the inner face of the membrane, consisting of a 25-repeat, rod-like, triple-helical domain separating an N-terminal actin binding domain from two C-terminal domains, one of which is rich in cysteine.

Imunogênio

recombinant human dystrophin fragment.

Aplicação

Monoclonal Anti-Dystrophin antibody produced in mouse has been used in

• immunohistochemistry
• immunofluorescence[1]
• double immunofluorescence terminal dUTP nick-end labeling (TUNEL)[1]
• immunoblotting

Monoclonal anti-dystrophin antibody can be used for localization of dystrophin using immunochemical assays like ELISA and immunohistochemistry. The antibody can also be used in immunoblotting for brain dystrophin. Further, it can be used in western blotting and double immunofluorescent labelling (diluted 1: 500) of dystrophin.

Ações bioquímicas/fisiológicas

Dystrophin deficiency is associated with severe Duchenne muscular dystrophy (DMD). Becker muscular dystrophy (BMD) show less pronounced abnormalities of dystrophin protein expression. Since abnormalities in the protein expression occur specifically in patients with these types of muscular dystrophy, dystrophin analysis may be used to distinguish these conditions from other neuromuscular diseases.

Descrição-alvo

The rod domain of the human dystrophin molecule is present in normal muscle tissue and in nearly all Becker muscular dystrophies. It is absent in the cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx).

Outras notas

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200764 Anti-Dystrophin antibody, Mouse monoclonal
clone MANDYS8, purified from hybridoma cell culture

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable