D2197
Diaphorase from Clostridium kluyveri
recombinant, expressed in E. coli, Animal-component free
Sinônimo(s):
Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Produtos recomendados
recombinante
expressed in E. coli
Nível de qualidade
forma
solid
atividade específica
>=30 units/mg protein (biuret)
temperatura de armazenamento
−20°C
Descrição geral
Research area: Cancer
Aplicação
Diaphorase from Clostridium kluyveri has been used:
- in lactate dehydrogenase assay in gill cells.
- impregnated on the strip for formate dehydrogenase assay.
- in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor.
- for semiquinone generation during benzoquinone reduction.
- for evaluation of glucose uptake in cultured muscle cells.
- in lactate dehydrogenase cytotoxicity assay.
Ações bioquímicas/fisiológicas
Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases that are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities.
Definição da unidade
One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.
forma física
Supplied as a lyophilized powder containing trehalose.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Apor Veres-Székely et al.
International journal of molecular sciences, 23(4) (2022-02-27)
Fibroblasts play a central role in diseases associated with excessive deposition of extracellular matrix (ECM), including idiopathic pulmonary fibrosis. Investigation of different properties of fibroblasts, such as migration, proliferation, and collagen-rich ECM production is unavoidable both in basic research and
Claudio F Gonzalez et al.
The Journal of biological chemistry, 280(24), 22590-22595 (2005-04-21)
Most bacteria contain soluble quinone-reducing flavoenzymes. However, no biological benefit for this activity has previously been demonstrated. ChrR of Pseudomonas putida is one such enzyme that has also been characterized as a chromate reductase; yet we propose that it is
Knut Erik Hovda et al.
Scandinavian journal of clinical and laboratory investigation, 75(7), 610-614 (2015-07-24)
The standard diagnostic approach to methanol poisoning is chromatographic measurement of methanol on centrally placed stationary equipment. Methanol poisoning in places where such equipment is unavailable is thus often not diagnosed. Methanol is metabolized to a toxic metabolite, formate; the
Transient Agarose Spot (TAS) Assay: A New Method to Investigate Cell Migration
Veres-Szekely A, et al.
International Journal of Molecular Sciences, 23(4), 2119-2119 (2022)
Relationship between insulin sensitivity and gene expression in human skeletal muscle
Parikh HM, et al.
BMC endocrine disorders, 21, 32-32 (2021)
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica