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CS0760

Sigma-Aldrich

Isolated Mitochondria Staining Kit

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

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1 KIT
R$ 4.515,00

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1 KIT
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About This Item

Número CE:
217-896-6
Código UNSPSC:
12352207
NACRES:
NA.32

R$ 4.515,00

Check Cart for Availability

Nível de qualidade

200

uso

 kit sufficient for 1000 reactions (using 96 multiwell plates)
 kit sufficient for 50 reactions (in a 2 mL cuvette)

embalagem

pkg of 1 kit

condição de armazenamento

dry at room temperature

técnica(s)

flow cytometry: suitable
protein staining: suitable

fluorescência

λex 490 nm; λem 590 nm (JC-1 dye)

aplicação(ões)

cell analysis
detection

método de detecção

fluorometric

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Categorias relacionadas

Aplicação

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.[1][2][3]

Características e benefícios

  • Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

Princípio

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

Nota de análise

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

Somente componentes do kit

Nº do produto
Descrição
  • DMSO 1 mL
  • JC-1 Assay Buffer 5X 25 mL
  • JC-1 Stain 25 μg
  • Valinomycin Ready Made 100 μL

Pictogramas

Skull and crossbones
GHS06

Palavra indicadora

Danger

Frases de perigo

H300 + H310

Classificações de perigo

Acute Tox. 1 Dermal - Acute Tox. 1 Oral

Código de classe de armazenamento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
0000275141
11180552
108M4080V
038M4176V
11171115

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