We have not tested collagen expressed in L929 cells, but we expect it to work.
CS0006
Soluble Collagen Quantification Assay Kit
Sinônimo(s):
Collagen Assay Kit
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.84
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
uso
sufficient for 200 fluorometric tests
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Categorias relacionadas
Descrição geral
Collagen is one of the most abundant proteins in connective tissues and internal organs of mammals. Collagen, a structural component, provides the tensile strength of the extracellular matrix (ECM) and is classified into several structurally and genetically distinct types. Although different types of collagen exist, they are all composed of molecules with three polypeptide chains that are arranged in a triple helical conformation. Slight differences in the primary structure (amino acid sequence) establish differences between the types.
The Soluble Collagen Quantification Assay Kit provides a simple and sensitive procedure for measuring soluble collagen in various sample types.
One of the advantages using our soluble collagen assay kit is that it does not require the use of the hazardous and corrosive perchloric acid as oppose to other kits in the market. Thus, it does not require a cumbersome and time-consuming procedure. You will get the result within 60 minutes: The amount of soluble collagen is determined based on an enzymatic reaction, where collagen is specifically digested into peptides. Subsequently, the collagen peptides are labeled with a fluorescent probe. The fluorescence intensity, measured at λ ex = 375 nm/ λ em = 465 nm, is proportional to the amount of soluble collagen in the sample.
This kit can be used to quantify soluble collagen extracted from soft tissues (e.g., muscle, heart, etc.), tissue culture cell lysate and medium, serum samples, collagen in food, and purified collagens of various sources. This kit can detect purified collagen types I, II, III, IV and V.
The detection range of the kit is 0.2-2 μg of soluble collagen.
The Soluble Collagen Quantification Assay Kit provides a simple and sensitive procedure for measuring soluble collagen in various sample types.
One of the advantages using our soluble collagen assay kit is that it does not require the use of the hazardous and corrosive perchloric acid as oppose to other kits in the market. Thus, it does not require a cumbersome and time-consuming procedure. You will get the result within 60 minutes: The amount of soluble collagen is determined based on an enzymatic reaction, where collagen is specifically digested into peptides. Subsequently, the collagen peptides are labeled with a fluorescent probe. The fluorescence intensity, measured at λ ex = 375 nm/ λ em = 465 nm, is proportional to the amount of soluble collagen in the sample.
This kit can be used to quantify soluble collagen extracted from soft tissues (e.g., muscle, heart, etc.), tissue culture cell lysate and medium, serum samples, collagen in food, and purified collagens of various sources. This kit can detect purified collagen types I, II, III, IV and V.
The detection range of the kit is 0.2-2 μg of soluble collagen.
Aplicação
Measuring soluble collagen in various sample types
Características e benefícios
Our economical Soluble Collagen Quantification Assay Kit also saves your time and efforts:
- Highly sensitive, simple and high-throughput assay for measuring soluble collagen in various samples
- User friendly: No need to weigh or calculate needed amounts of reagents on your own
- Customer safety: No need to use hazardous materials like strong acids, unlike other kits on the market which are based on hydroxyproline analysis.
- Supportive calculator (Click here to download a calculator excel file):Will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data.
- Quick instruction bench card - to assure your experimental success
- More experiments in one kit - contains enough reagents for 200 tests.
- Detection time: only 60 minutes
Adequação
Suitable for the detection of Soluble Collagen various biological samples and cultured cell medium.
Código de classe de armazenamento
10 - Combustible liquids
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Dongxu Feng et al.
The Journal of biological chemistry, 295(39), 13640-13650 (2020-08-05)
Progranulin (PGRN) is an autocrine growth factor that exerts crucial roles within cartilage tissue; however, the molecular mechanisms underlying PGRN-mediated cartilage homeostasis remain elusive. In the present study, we investigated the role of PGRN in regulating chondrocyte homeostasis and its
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Whether soluble collagen quantification assay KIT (CS0006) can test the collagen expressed from mouse L929 cells?
1 answer-
Helpful?
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Can this kit quantify soluble collagen peptides that don't possess hydroxyprolines?
1 answer-
Yes, this product will be suitable. The mechanism is not dependent on the presence of hydroxyproline.
Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/239/884/cs0006pis-ms.pdfHelpful?
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