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Sigma-Aldrich

CelLytic™ MEM Protein Extraction Kit

Sinônimo(s):

Membrane protein extraction kit

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About This Item

Código UNSPSC:
41116134
NACRES:
NA.56

técnica(s)

protein extraction: suitable

Nível de qualidade

200

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Categorias relacionadas

Descrição geral

CelLytic™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.
Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.

Aplicação

CelLytic™ MEM Protein Extraction Kit has been used for de novo lipogenesis measurements using hepatocytes and to extract membrane proteins for western blotting.

Adequação

Sufficient reagents supplied for 80 tests.

Informações legais

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Somente componentes do kit

Nº do produto
Descrição
  • Lysis and Separation Buffer 50 mL
  • Wash Buffer for CelLytic MEM 50 mL
  • Sodium Chloride, 4M Solution 1.5 mL

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mLSDS

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H315,H319

Classificações de perigo

Eye Irrit. 2 - Skin Irrit. 2

Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

188.6 °F - closed cup

Ponto de fulgor (°C)

87 °C - closed cup

Certificados de análise (COA)

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Willy Morelle et al.
The Journal of clinical endocrinology and metabolism, 102(4), 1375-1386 (2017-03-23)
TMEM165 deficiency is a severe multisystem disease that manifests with metabolic, endocrine, and skeletal involvement. It leads to one type of congenital disorders of glycosylation (CDG), a rapidly growing group of inherited diseases in which the glycosylation process is altered.
Sushmitha Vijaya Kumar et al.
Scientific reports, 10(1), 14985-14985 (2020-09-13)
Membrane organization plays an important role in signaling, transport, and defense. In eukaryotes, the stability, organization, and function of membrane proteins are influenced by certain lipids and sterols, such as cholesterol. Bacteria lack cholesterol, but carotenoids and hopanoids are predicted
Tomoaki Furuta et al.
Cancer science, 112(9), 3722-3731 (2021-06-12)
The rBC2LCN lectin, known as a stem cell marker probe that binds to an H type 3 fucosylated trisaccharide motif, was recently revealed to also bind to pancreatic ductal adenocarcinoma (PDAC) cells. A lectin-drug conjugate was generated by fusing rBC2LCN
Hiroaki Tateno et al.
Glycobiology, 26(12), 1328-1337 (2016-04-03)
Human somatic stem cells such as human mesenchymal stem cells (hMSCs) are considered attractive cell sources for stem cell-based therapy. However, quality control issues have been raised concerning their safety and efficacy. Here we used lectin microarray technology to identify
Elisabeth P Carpenter et al.
Current opinion in structural biology, 18(5), 581-586 (2008-08-05)
Membrane protein structural biology is still a largely unconquered area, given that approximately 25% of all proteins are membrane proteins and yet less than 150 unique structures are available. Membrane proteins have proven to be difficult to study owing to
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