71772
ProteoExtract® Transmembrane Protein Extraction Kit
Sinônimo(s):
Membrane Protein Isolation Kit
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About This Item
Código UNSPSC:
41106500
NACRES:
NA.77
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fabricante/nome comercial
Novagen®
condição de armazenamento
OK to freeze
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
Descrição geral
The ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).
Aplicação
Características e benefícios
- No sonication, intensive vortexing, lengthy ultracentrifugation, or high-temperature incubation is needed.
- Reagents are sufficient to process a total of 40 samples.
Advertência
Toxicity: Toxic (F)
Outras notas
Nakamura, E., et al. 2008. Cancer Sci.99, 1390.
Informações legais
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Skin Irrit. 2
Código de classe de armazenamento
10 - Combustible liquids
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