BF-11

Reactivity: Anti-Erythropoietin, human, 95050413

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: Rat x Mouse Hybridoma
• ImmunoDon: Rat AC1 spleen cells
• Mieloma: Sp2/0 Ag-14
• Reatividade: Anti-Erythropoietin, human
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat ACI spleen x Mouse Sp2/0 Ag-14

Descrição de linhagem celular

The BF-11 hybridoma cell line secretes a rat monoclonal antibody named Epoclonalan that reacts with human erythropoietin. Spleen cells from ACI strain rats that had been immunised with human urinary erythropoietin have been fused with Sp2/0 Ag-14 to produce this cell line. The α, β and asialo forms of human erythropoietin are bound by the antibody to the same extent.

Imunogênio

Human erythropoietin

Aplicação

Immunological studies, isolation of erythropoietin by immuno-affinity methods.

The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

Meio de cultura

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Rotina de subcultura

Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO₂; 37°C.

Outras notas

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