HT2 Clone A5E

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92021401, mouse blood, Lymphoblast

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: mouse blood
• embalagem: tube of 5 μg 92021401-DNA-5UG; pkg of vial of cells 92021401-1VL
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblast
• produtos: Not specified
• receptores: Interleukin 2 (IL-2), Interleukin 4 (IL-4)
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse helper T cell

Descrição de linhagem celular

Derived in 1979 from BALB/c spleen cells activated by sheep erythrocytes in the presence of IL-2, the A5E clone was selected to provide a homogenous population that is highly sensitive to IL-2. This cell line expresses Thy-1 and Lyt markers in addition to high affinity IL-2 receptors. Substantial proliferation is seen in the presence of IL-4 (GM-CSF).

Aplicação

IL-2 assays

Meio de cultura

RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 100-200 IU/ml IL-2 + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. When starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g

Outras notas

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