Detroit 510

90102531, human skin, Fibroblast

• Código UNSPSC: 41106514

Propriedades

• product name: Detroit 510, 90102531
• fonte biológica: human skin
• modo de crescimento: Adherent
• cariótipo: 2n = 46
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian skin Galactosemia

Descrição de linhagem celular

Derived from skin tissue of a 9 month old Caucasian female with Galactosemia. Galactose-1-phosphate transferase negative.

Aplicação

Virus studies

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 10,12
D7S820: 8,12
THO1: 7,9.3
TPOX: 8
vWA: 15,17

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10%Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Finite lifespan, up to 30 population doublings are expected.

Outras notas

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