PLC/PRF/5

85061113, human liver, epithelial

• Sinônimo(s): Alexander cell line
• Código UNSPSC: 41106514

Propriedades

• product name: PLC/PRF/5, from human liver, 85061113
• fonte biológica: human liver
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: epithelial
• produtos: Hepatitis B Surface Antigen (HBSAg)
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição de linhagem celular

Alexander cell line which produces Hepatitis B antigen. The virus genome may be inducible in selective media. The cells should be handled under laboratory containment level 2. Expresses c-abl, c-fes, c-fms, c-myc, c-ha-ras and c-sis oncogenes. However there is currently no evidence that this cell line produces infectious Hepatitis B virus.

Perfil do DNA

STR-PCR Data:

Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 9,11
THO1: 7,8
TPOX: 8
vWA: 15,16

Meio de cultura

DMEM + 2mM Glutamine + 10% fetal bovine serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6; i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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