CCRF-SB

89090405, human blood, Lymphoblastoid

• Código UNSPSC: 41106514

Propriedades

• product name: CCRF-SB, 89090405
• fonte biológica: human blood
• modo de crescimento: Suspension
• cariótipo: 2n = 46
• morfologia: Lymphoblastoid
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian acute lymphoblastic leukaemia

Descrição de linhagem celular

A B lymphoblastoid line obtained from the peripheral blood of an 11 year old boy with acute lymphoblastic leukaemia. Appear free of virus particles by electron microscopy. Do not synthesise immunoglobulins. Grown in continuous spinner culture for 6 months. HLA profile: A1, A2, B12, B17, Cw2.

Aplicação

HLA profile studies

Meio de cultura

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 3x10,000 to 1x100,000 cells/ml; 5% CO₂; 37°C. Cultures can tolerate high densities i.e. 1-2x1,000,000 cells/ml.

Outras notas

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