10021102

Bob cell line human

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human prostate
• Nível de qualidade: 100
• Formulário: liquid
• embalagem: tube of 5 μg 10021102-DNA-5UG; pkg of vial of cells 10021102-1VL
• modo de crescimento: Adherent
• cariótipo: 4n (81 to 94)
• morfologia: Cobblestone
• produtos: 10% 3-D sphere formation in Matrigel. Migratory and invasive when compared to benign prostate tumour.
• receptores: CD44, alpha2 integrin, alpha6 integrin, EGFR, FGFR1, LIFR
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer

Descrição

Origem de linhagem celular

Human prostate cancer spontaneously immortalized, serum-free

Descrição de linhagem celular

First spontaneously immortalized prostate cancer cell line to be established from a trans-rectal needle biopsy (TRBP) of a patient with castration-resistant prostate cancer (CRPC). Bob is a novel pre-clinical model for functional studies in CRPC and especially for studying the CRPC "basal" phenotype. This cell line is cultured in serum-free culture medium, a derivative of Bob is also available i.e. SerBob, Sigma Catalogue number 10021101, which has been adapted to medium containing serum. SerBob is more differentiated and invasive than Bob. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Aplicação

For complete product information, please see ECACC

Prostate cancer research including drug testing.

Embalagem

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,14
D16S539: 10,12
D5S818: 13
D7S820: 10,11
THO1: 9
TPOX: 8,10
vWA: 17

Meio de cultura

Keratinocyte-SFM supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor, 2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2-4 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells must be centrifuged at 400 x g for 5 minutes to remove the trypsin at each subculture. Cells are cryopreserved in 45% conditioned media: 45% fresh media: 10% DMSO. At confluence 5-7 x 104 cells/cm² can be expected.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable