GH1

89072610, rat brain, Epithelial

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: GH1, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 89072610
• fonte biológica: rat brain
• embalagem: tube of 5 μg 89072610-DNA-5UG; pkg of vial of cells 89072610-1VL
• modo de crescimento: Semi-adherent
• cariótipo: Hypodiploid, modal no. 40.
• morfologia: Epithelial
• produtos: Rat growth hormone, lactogenic hormones.
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Rat Wistar-Furth pituitary tumour

Descrição de linhagem celular

Derived from pituitary gland of Wistar-Furth rats. The cells secrete growth hormone at a rate of 1μg/ml cell protein/day. Re-inoculation of cloned cells back to Wistar-Furth rats results in a marked increase in body weight and stimulation of linear growth in both intact and hypophysectomized animals independent of rat tumour size.

Aplicação

Hormone studies

Meio de cultura

Ham′s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. These cells only semi-attach and may return to suspension during transit. If this has occurred on receipt of the cells pellet cells b

Outras notas

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