HeLa B

85060701, human cervix, Epithelial

• Código UNSPSC: 41106514

Propriedades

• product name: HeLa B, 85060701
• fonte biológica: human cervix
• modo de crescimento: Adherent
• cariótipo: Aneuploid
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human cervix carcinoma

Descrição de linhagem celular

A derivative of HeLa, the first aneuploid, epithelial-like line derived from human tissue and maintained by serial subculture. Identified as a contaminant in many subsequently derived lines, due to poor culture techniques. Susceptible to poliovirus 1 and Ad 3.

Aplicação

Tumourigenicity and virus studies: poliovirus 1 and Ad 3

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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