This product is provided as a solution with a minimum concentration of 0.50 mg protein/mL Carboxypeptidase B in 0.05 M NaOAc pH 5.0, containing 1.0 M NaCl and 0.01% NaN3. The concentration is lot-specific and reported in the product Certificate of Analysis. The maximum volume, based on the product specification, is 0.2 mL. This product is stored at −20°C and ships on dry ice. Please see the link below to review a sample or lot-specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/sigma/c5233#product-information
C5233
Carboxypeptidase B from human pancreas
50-55 units/mg protein carboxypeptidase B
Sinônimo(s):
Peptidyl-L-Lysine[L-arginine] hydrolase
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About This Item
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fonte biológica
human pancreas
Nível de qualidade
Formulário
solution
atividade específica
50-55 units/mg protein carboxypeptidase B
Impurezas
≤0.2% chymotrypsin
≤0.2% trypsin
≤1 unit/mg protein carboxypeptidase A
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
cadeia de caracteres SMILES
S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
chave InChI
TWURVFFNODFJBJ-UHFFFAOYSA-N
Informações sobre genes
human ... CPB1(1360)
Categorias relacionadas
Descrição geral
Aplicação
Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat.[3] The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.[4]
Ações bioquímicas/fisiológicas
Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34,000 Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro.[5] The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.
Definição da unidade
One unit will hydrolyze 1 μmole of hippuryl-L-arginine per minute at pH 7.7 at 25 °C
forma física
Solution in 0.05 M NaOAc pH 5.0 + 1.0 M NaCl + 0.01% NaN3
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Certificados de análise (COA)
Lot/Batch Number
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E Sim et al.
The Biochemical journal, 239(3), 763-767 (1986-11-01)
The plasma complement protein C4 is encoded at two highly polymorphic loci, A and B, within the class-III region of the major histocompatibility complex. At least 34 different polymorphic variants of human C4 have been identified, including non-expressed or 'null'
Combined proteomics and transcriptomics identifies carboxypeptidase b1 and nuclear factor kappaB (NF-kappaB) associated proteins as putative biomarkers of metastasis in low grade breast cancer
Bouchal P, et al.
Molecular and Cellular Proteomics, 14(7), 1814-1830 (2015)
Xiu Qin Xu et al.
The Journal of biological chemistry, 280(25), 23987-24003 (2005-04-23)
We have exploited a discrepancy in the oncogenic potential of autocrine and exogenous human growth hormone (hGH) in an attempt to identify molecules that could potentially be involved in oncogenic transformation of the human mammary epithelial cell. Microarray analysis of
Mutations in the pancreatic secretory enzymes CPA1 and CPB1 are associated with pancreatic cancer
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Proceedings of the National Academy of Sciences of the USA, 201720588-201720588 (2018)
Lawrence L K Leung et al.
Advances in experimental medicine and biology, 632, 61-69 (2008-11-26)
Thrombin-activatable procarboxypeptidase B (proCPB or thrombin-activatable fibrinolysis inhibitor or TAFI) is a plasma procarboxypeptidase that is activated by the thrombin-thrombomodulin complex on the vascular endothelial surface. The activated CPB removes the newly exposed carboxyl terminal lysines in the partially digested
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I have a question about the Carboxypeptidase B (C-5233). Generally, how much volume of buffer solution (0.05M NaOAc pH 5.0+1.0M NaCl+0.01% NaN3) to add to make the stock? What is the storage temp. for the stock solution after reconstitution? Thank you!
1 answer-
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