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C1117

Sigma-Aldrich

Anti-Cy5 antibody, Mouse monoclonal

clone CY5-15, purified from hybridoma cell culture

Sinônimo(s):

Cy5 Antibody, Cy5 Antibody - Anti-Cy5 antibody, Mouse monoclonal, Monoclonal Anti-Cy5 antibody produced in mouse

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About This Item

Número MDL:
MFCD08705581
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

200

conjugado

unconjugated

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

CY5-15, monoclonal

forma

buffered aqueous solution

embalagem

antibody small pack of 25 μL

concentração

~1.5 mg/mL

técnica(s)

direct ELISA: suitable
dot blot: 1-2 μg/mL using cell protein extracts labeled with Cy5
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable

Isotipo

IgG1

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Descrição geral

CyDyes are a family of fluorophores that can be used for labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are small, pH insensitive (between pH 3 and pH 10), and soluble in aqueous solution and have tolerance to DMSO. They are more photo-stable than fluorescein, have high molar extinction coefficients, and favorable quantum yields. Due to their physical and chemical properties, they are used (mainly Cy3 and Cy5) in many different biological assays such as: DNA microarrays, protein microarrays, two-dimensional protein analysis (2D gels), fluorescence resonance energy transfer (FRET), and immunocytochemistry.
Mouse monoclonal clone CY5-15 anti-Cy5 antibody recognizes Cy5 and AlexaFluor647 labeled proteins, but not Cy3 labeled proteins.

Especificidade

Monoclonal Anti-Cy5 recognizes Cy5 and AlexaFluor647 labeled proteins, but not Cy3 labeled proteins.
The antibody recognizes Cy5 and AlexaFluor 647 labeled proteins, but not Cy3 labeled proteins.

Imunogênio

mixture of proteins labeled with Cy5.

Aplicação

Anti-Cy5 antibody, Mouse monoclonal has been used in immunofluorescence assays and western blotting.
Mouse monoclonal clone CY5-15 anti-Cy5 antibody is used to tag CyDye 5 for detection and quantitation and signal applification by or within immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoprecipitation, immunocytochemistry, dot blot assay, and protein microarrays. Anti-Cy5 antibody is an important tool for Cy5 signal amplification in assays where biomolecules are labeled with Cy5.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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