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Sigma-Aldrich

Annexin V-Cy3™ Apoptosis Detection Kit

Sinônimo(s):

Red fluorescent Annexin V apoptosis assay

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.32

uso

sufficient for 200 tests

Nível de qualidade

200

embalagem

pkg of 1 kit

técnica(s)

flow cytometry: suitable

aplicação(ões)

cell analysis
detection

método de detecção

fluorometric

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Características e benefícios

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Princípio

Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Informações legais

Cy3 is a trademark of Cytiva

Somente componentes do kit

Nº do produto
Descrição
  • Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS
  • C50416-Carboxyfluorescein diacetate 10 mgSDS

Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Kasturi Muthoosamy et al.
Scientific reports, 6, 32808-32808 (2016-09-07)
Metastasis of lung carcinoma to breast and vice versa accounts for one of the vast majority of cancer deaths. Synergistic treatments are proven to be the effective method to inhibit malignant cell proliferation. It is highly advantageous to use the
Christo Paul Preethy et al.
Journal of pharmacology & pharmacotherapeutics, 3(1), 26-34 (2012-03-01)
To find the efficacy of serial extracts of Anisomeles malabarica in inhibiting proliferation of and inducing apoptosis in human cervical cancer cells, SiHa and ME 180, that are HPV 16-positive. The whole plant was extracted in n-hexane, chloroform, ethyl acetate
Rajeev Kumar Pandey et al.
PloS one, 6(2), e17093-e17093 (2011-02-25)
Mycobacterium indicus pranii (MIP), also known as Mw, is a saprophytic, non-pathogenic strain of Mycobacterium and is commercially available as a heat-killed vaccine for leprosy and recently tuberculosis (TB) as part of MDT. In this study we provide evidence that
Barbara Sorce et al.
Nature communications, 6, 8872-8872 (2015-11-26)
Little is known about how mitotic cells round against epithelial confinement. Here, we engineer micropillar arrays that subject cells to lateral mechanical confinement similar to that experienced in epithelia. If generating sufficient force to deform the pillars, rounding epithelial (MDCK)
Anvarbatcha Riyasdeen et al.
Evidence-based complementary and alternative medicine : eCAM, 2012, 136527-136527 (2011-11-25)
Rasagenthi Mezhugu (RGM) is a herbomineral formulation in the Siddha system of traditional medicine and is prescribed in the southern parts of India as a remedy for all kinds of cancers. However, scientific evidence for its therapeutic efficacy in cervical
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Artigos

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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