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A8311

Sigma-Aldrich

Antiespumante 204

aqueous emulsion for bacterial and mammalian systems

Sinônimo(s):

organic antifoam

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About This Item

Número MDL:
Código UNSPSC:
12161901
NACRES:
NA.85

Nível de qualidade

forma

emulsion (aqueous)

concentração

100% (organic)

densidade

1.01 g/mL at 25 °C

aplicação(ões)

microbiology

temperatura de armazenamento

room temp

adequação

(Mammalian (suspension))
bacteria (fermentation)

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Descrição geral

Antifoam 204 is an organic antifoam used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Aplicação

Suitable for preventing or minimizing foaming in microbial fermentation systems and mammalian suspension cultures.
Antifoam 204 has been used:
  • in the expression and purification of PUL (PLAP, Ufd3p, and Lub1p) domain
  • in fed-batch fermentation
  • to prevent bubble formation due in aeration in Luria Bertani (LB) culture medium for bacterial growth
  • in the batch fermentation with mineral medium

Componentes

Antifoam 204 is a 100% active components and a mixture of non-silicone organic defoamers in a polyol dispersion.

Outras notas

Contém componentes 100% ativos e é uma mistura de antiespumantes orgânicos não siliconados em uma dispersão de poliol. Pode ser repetidamente esterilizado.

Nota de preparo

For use in microbiological media Sigma recommends a starting concentration of between 0.005% and 0.01%.
The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol.

produto relacionado

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves


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