A7510
Adenosine 5′-Triphosphatase from porcine cerebral cortex
lyophilized powder, ≥0.3 units/mg protein, pH 7.8
Sinônimo(s):
ATP phosphohydrolase, ATPase
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About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
Preço e disponibilidade não estão disponíveis no momento.
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fonte biológica
Porcine cerebral cortex
Nível de qualidade
descrição
Sodium and Potassium-Activated
Formulário
lyophilized powder
atividade específica
≥0.3 units/mg protein, pH 7.8
composição
Protein, 5.0-25.0% biuret
técnica(s)
inhibition assay: suitable
temperatura de armazenamento
−20°C
Informações sobre genes
pig ... ATP1A1(397481) , ATP1B1(396898)
Aplicação
ATPase is used to liberate inorganic phosphorus from ATP. ATPase, from porcine cerebral cortex, has been used in inhibition assays of Na/K-ATPase activity [1].
Ações bioquímicas/fisiológicas
This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA).
This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). Bufalin, and other cardiotonic steroids, may inhibit Na+/K+-ATPase activity and induce the differentiation of HL60, U937, and ML1 cells [1].
Definição da unidade
One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 7.8 at 37 °C in the presence of Na+, K+, and Mg2+.
forma física
Lyophilized powder containing 90% sucrose, 0.4% EDTA Na4 and 0.06% NaCl
Nota de preparo
Sodium iodide extracted fraction of Nakao, et al., Biochem. Biophys. Res. Commun., 19, 755 (1965).
Inibidor
Nº do produto
Descrição
Preços
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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