A7419
Avidin–Peroxidase
buffered aqueous solution
Sinônimo(s):
Avidin–HRP
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2 ML
R$ 2.895,00
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2 ML
R$ 2.895,00
About This Item
Produtos recomendados
fonte biológica
avidin from egg white
enzyme from horseradish
conjugado
peroxidase conjugate
Formulário
buffered aqueous solution
técnica(s)
indirect ELISA: 1:100,000
western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Descrição geral
Avidin is homotetrameric protein (68kDa) obtained from egg whites and binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Aplicação
Avidin-Peroxidase has been used for ELISA applications[2][3]. The product can also be used for western blot applications at dilutions ranging from 1:400,000 to 1:800,000.
Western blot analysis of proteins in HUVEC cells that had underwent cell surface biotinylation were detected using HRP-conjugated avidin at 33.3 ng/ml. [4]
forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.
Nota de preparo
Affinity purified protein.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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