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A2418

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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1 ML
R$ 2.990,00

R$ 2.990,00

Previsão de entrega em16 de abril de 2025

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1 ML
R$ 2.990,00

About This Item

Número MDL:
MFCD00162640
Código UNSPSC:
12352203
NACRES:
NA.46

R$ 2.990,00

Previsão de entrega em16 de abril de 2025

fonte biológica

rabbit

Nível de qualidade

200

conjugado

alkaline phosphatase conjugate

forma do anticorpo

IgG fraction of antiserum

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

Formulário

buffered aqueous solution

reatividade de espécies

mouse

técnica(s)

direct ELISA: 1:15,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tonsil tissue
western blot (chemiluminescent): 1:40,000-1:80,000 using deecting β-Actin in total cell extract of HeLa cells (5-10 μg/mL)

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

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Descrição geral

IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3.

Imunogênio

purified mouse IgG

Aplicação

Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in rabbit has been used in :
  • direct enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunohistochemistry

Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.

Ações bioquímicas/fisiológicas

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG antibody participates in complement fixation and opsonization.

forma física

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
127M4803V
077M4759V
065M4782V
044M4751

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Guangyi Qin et al.
Oncology letters, 15(6), 8756-8760 (2018-05-29)
The present study aimed to investigate the effect of allyl isothiocyanate (AITC) on the viability and apoptosis of the human cervical cancer HeLa cell line in vitro, and to explore the potential underlying mechanisms of this. HeLa cells were treated
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Structure and function of human and murine receptors for IgG
Unkeless J C, et al.
Annual Review of Immunology, 6(1), 251-281 (1988)
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Transgenic research, 11(5), 479-493 (2002-11-20)
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S El-Amouri et al.
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Obesity-associated diabetes is linked to the accumulation of ceramide in various organs, including the liver. The exact mechanisms by which ceramide contributes to diabetic pathology are unclear, but one proposed scenario is that ceramide accumulation may inhibit insulin signaling pathways.
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