89524

Azide cyanine dye 728

for copper catalyzed click labeling

• Sinônimo(s): 2-{2-{2-[(3-Azidopropyl)amino]-3-{2-[1,3-dihydro-3,3-dimethyl-1-(4-sulfobutyl)-2H-indol-2-ylidene]ethylidene}-1-cyclopenten-1-yl}ethenyl}-3,3-dimethyl-1-(4-sulfobutyl)-3H-indolium inner salt
• Fórmula empírica (Notação de Hill): C₄₀H₅₂N₆O₆S₂
• Número CAS: 1188332-22-0
• Peso molecular: 777.01
• Número MDL: MFCD18452836
• Código UNSPSC: 12352200
• ID de substância PubChem: 329769746
• NACRES: NA.32

Propriedades

• Formulário: powder
• fluorescência: λ_ex 670 nm; λ_em 728 nm in ethanol
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: CC1(C)\C(=C/C=C2\CCC(\C=C\C3=[N+](CCCCS([O-])(=O)=O)c4ccccc4C3(C)C)=C2NCCCN=[N+]=[N-])N(CCCCS(O)(=O)=O)c5ccccc15
• InChI: 1S/C40H52N6O6S2/c1-39(2)32-14-5-7-16-34(32)45(26-9-11-28-53(47,48)49)36(39)22-20-30-18-19-31(38(30)42-24-13-25-43-44-41)21-23-37-40(3,4)33-15-6-8-17-35(33)46(37)27-10-12-29-54(50,51)52/h5-8,14-17,20-23H,9-13,18-19,24-29H2,1-4H3,(H2,47,48,49,50,51,52)/b30-20+,36-22+
• chave InChI: XEWMTEFQJKJRAZ-TXRIKVQFSA-N

Descrição

Descrição geral

The MegaStokes dyes for „click" biological labeling, licensed by LuminoChem are virtually covering the whole visible spectrum reaching the infra-red regime. Besides dyes that are being capable of participating in classical copper catalyzed 1,3-dipolar cycloaddition reaction with the counterparting function, we also provide dyes containing cyclooctyne moiety an alkyne derivative that enables copper free clicking to azides.
These dyes are noteworthy for their large Stokes-shift (120 or 125 nm). Dyes for this kind are exceptionally useful in fluorescence resonance electron transfer (FRET) applications as they do not interfere with the spectral bands of the second fluorophore a common problem in FRET technology. Azido sugars have been used for click-labeling of surface glycoproteins, recently. We have adapted this system to demonstrate the ability of our dyes to undergo bioorthogonal labeling reaction, efficiently. Prior to labeling, Chinese hamster ovary (CHO) cells were incubated with these MegaStockesdyes to test the possible cytostatic effects of these dyes. Experimental results have shown that neither dye is toxic up to 50 μM concentration.

Embalagem

Bottomless glass bottle. Contents are inside inserted fused cone.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H315,H319,H335
• Declarações de precaução: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable