88021103
Clone 1-5c-4 cell line (HeLa deriative)
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About This Item
Código UNSPSC:
41106514
Produtos recomendados
fonte biológica
human cervix
forma
liquid
modo de crescimento
Adherent
cariótipo
2n = 46
morfologia
Epithelial
produtos
Not specified
receptores
Not specified
técnica(s)
cell culture | mammalian: suitable
doença(s) relevante(s)
cancer
Condições de expedição
dry ice
temperatura de armazenamento
−196°C
Origem de linhagem celular
Human cervix carcinoma
Descrição de linhagem celular
These cells were originally Chang conjunctival cells adapted by Wong and Kilbourne, and designated as conjunctival ′D′ cells. Susceptible to swine influenza viruses, supports production of plaque formation by a variety of influenza virus subtypes. Has HeLa markers, exhibits type A G6PD. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Epithelial with occasional giant cells. Ethnicity: Black.
Aplicação
Plaque assays, cloning, genetic and virus studies: swine influenza viruses
Meio de cultura
Medium 199 (HBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Rotina de subcultura
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using EDTA or 0.25% trypsin; 5% CO2; 37°C.
Outras notas
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Exoneração de responsabilidade
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Nº do produto
Descrição
Preços
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
K S Lavappa
In vitro, 14(5), 469-475 (1978-05-01)
Seed stocks of human cell lines deposited at the American Type Culture Collection (ATCC) have been examined for cross-contamination with HeLa cells using Giemsabanded marker chromosomes. Sixteen additional cell lines investigated have been found to exhibit marker chromosomes typical of
Continuous subcultivation of epithelial-like cells from normal human tissues.
R S M CHANG
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 87(2), 440-443 (1954-11-01)
Examination of ATCC stocks for HeLa marker chromosomes in human cell lines.
K S Lavappa et al.
Nature, 259(5540), 211-213 (1976-01-22)
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