79636

Atto 590 NHS ester

BioReagent, suitable for fluorescence, ≥90.0% (degree of coupling)

• Fórmula empírica (Notação de Hill): C₄₁H₄₂ClN₃O₁₁
• Número CAS: 670269-33-7
• Peso molecular: 788.24
• Número MDL: MFCD03456133
• Código UNSPSC: 12352108
• NACRES: NA.32

Propriedades

• linha de produto: BioReagent
• Ensaio: ≥90.0% (HPLC); ≥90.0% (degree of coupling)
• Formulário: powder
• fabricante/nome comercial: ATTO-TEC GmbH
• λ: in ethanol (with 0,1% trifluoroacetic acid)
• Absorção UV: λ: 597-663 nm A_max
• adequação: suitable for fluorescence
• método de detecção: fluorometric
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: [Cl](=O)(=O)(=O)[O-].[N+]1(=C2C(=CC3=C(c6c(cc7c(c6)C(=CC(N7CC)(C)C)C)OC3=C2)c4c(ccc(c4)C(=O)ON5C(=O)CCC5=O)C(=O)O)C(=CC1(C)C)C)CC
• InChI: 1S/C41H41N3O7.ClHO4/c1-9-42-31-18-33-29(16-26(31)22(3)20-40(42,5)6)37(30-17-27-23(4)21-41(7,8)43(10-2)32(27)19-34(30)50-33)28-15-24(11-12-25(28)38(47)48)39(49)51-44-35(45)13-14-36(44)46;2-1(3,4)5/h11-12,15-21H,9-10,13-14H2,1-8H3;(H,2,3,4,5)
• chave InChI: SHJDVERDESTYRQ-UHFFFAOYSA-N

Descrição

Descrição geral

Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. The Atto dyes are a series of fluorescent dyes that provide all the crucial properties required for modern fluorescent technologies, such as fluorescence microscopy, flow-cytometry, fluorescence in situ hybridization (FISH), receptor binding assays or enzyme assays. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy. The NHS-esters are used in common conjugation protocols.

Aplicação

Atto 590 NHS ester may be suitable for use in site-specific labeling of human embryonic kidney (HEK293T) cell lysates for western blotting, fluorescence, and widefield microscopy studies.[1]

Molar absorption 120,000 1/M cm, abs: 593 nm, em: 620 nm, QY=0.93, T_fl 4.0 ns (unpublished data)

Características e benefícios

• Absorption maxima cover a range from 590 to 620 nm.
• Structural properties support extraordinary photostability.
• High fluorescence quantum yield and high thermal stability.
• The fluorescence characteristics of the dye are quite insensitive to environmental changes (e.g., pH changes). This holds for both excitation and emission wavelengths and for the decay time of their fluorescence emission.
• Compatible with common labeling procedures for proteins and amino-functionalized oligonucleotides.

Outras notas

Study of multistep energy transfer in a single photonic wire by FRET[2]; employed in fluorescence energy transfer, FRET, scanning microscopy[3].

Informações legais

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)