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64958

Sigma-Aldrich

2-Methoxy-2,4-diphenyl-3(2H)-furanone

suitable for fluorescence, ≥98.0% (HPLC)

Sinônimo(s):

2,4-Diphenyl-2-methoxy-3(2H)-furanone, MDPF

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25 MG
R$ 1.837,00
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About This Item

Fórmula empírica (Notação de Hill):
C17H14O3
Número CAS:
50632-57-0
Peso molecular:
266.29
Beilstein:
1288529
Número MDL:
MFCD00036834
Código UNSPSC:
12352108
ID de substância PubChem:
329760016
NACRES:
NA.32

R$ 1.837,00

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Nível de qualidade

100

Ensaio

≥98.0% (HPLC)

Formulário

solid

fluorescência

λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)

adequação

suitable for fluorescence

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

COC1(OC=C(C1=O)c2ccccc2)c3ccccc3

InChI

1S/C17H14O3/c1-19-17(14-10-6-3-7-11-14)16(18)15(12-20-17)13-8-4-2-5-9-13/h2-12H,1H3

chave InChI

BLWINLJDTOJSRU-UHFFFAOYSA-N

Aplicação

2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.

Outras notas

Reagent for the derivatization of primary and secondary amines for HPLC; highly fluorescent derivatives are formed[1][2]; Pre-column derivatization of amines[3]; Fluorescent labeling of proteins before SDS-PAGE[4]

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H315,H319,H335

Classificações de perigo

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Gloves

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Certificados de análise (COA)

Lot/Batch Number
BCCL1534
BCBT5586
BCBS6087V
BCBP1051V
BCBK6273V

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R C Schaeffer et al.
International journal of microcirculation, clinical and experimental, 11(2), 181-201 (1992-05-11)
We studied the macromolecular size-selective transport characteristics of polycarbonate (PC) filters with defined pore radius (rp; 15,000 to 400 A) as well as 2,000 A rp PC filter-bovine pulmonary artery endothelial cell (EC) monolayer sandwich under zero hydrostatic pressure conditions
S K Williams et al.
Journal of cellular physiology, 120(2), 157-162 (1984-08-01)
The transport of proteins across continuous capillary endothelium is believed to be mediated by micropinocytic vesicles which shuttle molecules between the lumenal and abluminal plasma membrane. We have studied the ability of capillary endothelial cells isolated from rat epididymal fat
B W Falk et al.
Analytical biochemistry, 144(2), 537-541 (1985-02-01)
Fluorescent labeling of proteins was found to be a very sensitive and reliable alternative to conventional methods for monitoring proteins on Western blots. Proteins were labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) before SDS-PAGE. After electrophoresis and subsequent electro-blotting the fluorescent-labeled proteins were
F J Alba et al.
Electrophoresis, 19(14), 2407-2411 (1998-11-20)
The fluorogenic dye 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) has been used for the detection of total protein patterns on polyvinylidene difluoride (PVDF) membranes. Fluorescent staining of protein bands on membranes with this covalent dye is completed in 20 min. Wet membranes are translucent
M Hengstschläger et al.
Cytometry, 14(1), 39-45 (1993-01-01)
Thymidine kinase is a key enzyme for the application of drugs in chemotherapy and for diagnosis. Although of great interest, its regulation during cell cycle and differentiation is difficult to study, as current techniques for isolation of cells in different
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