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Documentos Principais

10072802

CCB cell line from carp

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

Código UNSPSC:
41106514
NACRES:
NA.81
Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

fish brain

embalagem

tube of 5 μg 10072802-DNA-5UG
pkg of vial of cells 10072802-1VL

modo de crescimento

Adherent

cariótipo

Not specified

morfologia

Squamous

produtos

Not specified

receptores

Not specified

técnica(s)

cell culture | mammalian: suitable

Origem de linhagem celular

Common carp brain cell line, koi herpesvirus isolation

Descrição de linhagem celular

The CCB cell line was developed from Common carp (Cyprinus carpio carpio) brain.

Embalagem

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Meio de cultura

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). 10 mM HEPES can be included if a CO2 incubator is not available.

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:6, i.e., seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 25 °C.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Lot/Batch Number

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Irene Cano et al.
International journal of molecular sciences, 21(22) (2020-11-15)
Seroconversion and the mucosal lysozyme G (lysG), complement 3 (c3), and immunoglobulins M (IgMsec) and Z2 (IgZ2) were measured for up to 900 degree days (DD) in skin swabs from common carp exposed to koi herpesvirus (KHV or CyHV-3) at
Irene Cano et al.
Animals : an open access journal from MDPI, 11(2) (2021-02-13)
Fluorescence real-time LAMP assays were designed for the orf43 gene of CyHV-3 European genotype and the p4a gene of the CEV genogroup I. A third LAMP assay to detect the ef1a gene of the host common carp was designed as
Agnieszka Troszok et al.
Journal of fish diseases, 41(11), 1709-1718 (2018-08-26)
Cyprinid herpesvirus 3 (CyHV-3), also known as koi herpesvirus (KHV), is an aetiological agent of a virulent and lethal disease in common and koi carp. In this study, we examined in vitro the anti-CyHV-3 activity of acyclovir (ACV), nucleoside analogue commonly

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