08061901

U-373 MG (Uppsala) cell line

• Sinônimo(s): U-373MG Cells, U373-MG Cells, U373MG Cells
• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human brain
• forma: liquid
• modo de crescimento: Adherent
• cariótipo: 2n = 46
• morfologia: Pleomorphic/astrocytoid
• receptores: PDGFR alpha and EGFR. See Nistér M. et al., (1988) & (1991)
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human glioblastoma astrocytoma

Descrição de linhagem celular

A human glioblastoma astrocytoma derived from a malignant tumour by explant technique. This catalogue entry represents a new deposit of U-373 MG obtained from the originator′s laboratory in Uppsala; it has been designated U-373 MG (Uppsala) to distinguish it from U-373 MG (Sigma Catalogue number 89081403) which is now known to be identical, by Short Tandem Repeat (STR)-PCR profiling, to another glioblastoma cell line, U-251 MG (please read below for more information about this discovery). U-373 MG (Uppsala) has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma.
Background to the identity query for the cell line U-373 MG: The American Type Culture Collection (ATCC) reported that their stock of U-373 MG had been shown to have differing genetic properties to stock from the originator′s laboratory, and to share similarities with another glioblastoma cell line, U-251. In light of this, ECACC undertook an investigation into the authenticity of its own stock of the U-373 MG cell line. ECACC found similar results to the ATCC i.e. the stock held as U-373 MG was found to be identical by STR-PCR profiling to U-251. The U-373 MG cell line listed under catalogue number 89081403 has been re-named as ′U-251 (formerly known as U-373 MG)′.

Aplicação

Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate + 10% Fetal Bovine Serum (FBS).

Ações bioquímicas/fisiológicas

U-373 MG (Uppsala) is a human glioblastoma astrocytoma derived from a malignant tumour by explant technique.

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 8
D16S539: 12,13
D5S818: 12
D7S820: 8.2,12
THO1: 7,9.3
TPOX: 8
vWA: 17,18

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable